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Guangfu Wang
Publisher:高雪  Time2017-09-14 View:252

 

Guangfu Wang


Phone: 86-451-86418677
E-mail:sharrum@163.comwangguangfu@hit.edu.cn
Research Area: Neuroscience


Guangfu Wang studied at the School of Electronics Engineering and Computer Science (EECS)  of  Peking University  (PKU), where he received a B.S degree in electronics and  a Ph.D. degree in radio physics in 2003 and 2008,  respectively. In the  nine years of study and research in PKU, Wang accumulated  solid knowledge in electronics, physics and computer science by accomplishing  various projects,  including precision current controller & temperature  controller for laser  diodes, holographic optical tweezers, and neutral atomic  optical frequency  standards. After graduating from PKU, he realized that his  true passion was interdisciplinary  study of physics and biology, and then  joined Julius Zhu lab in University of  Virginia (UVa) for neuroscience study.  In the following years, Dr. Wang  overcame various mechanical, electronic,  optical and software barriers, and  independently built up an experimental setup  featuring octuple whole-cell  recording, two-photon imaging and one-/two-photon  optogenetic stimulation. So far, using this setup, Dr. Wang and colleagues have accomplished  several projects related to neuronal  circuitry and synaptic function, and  published the results in high-ranked journals.  They  discovered two layer 1 (L1) neuron-led disinhibitory and  inhibitory neuronal  circuits that control the initiation of the dendritic  potentials. They found  that these two circuits act synergistically with the  dendritic coincidence  mechanism to achieve salience selection. This body of  work deciphers the  organization of cortical L1 neuron-led transsynaptic  circuits at cellular and  subcellular levels. It reveals the essential role of  the interneuronal  circuits in governing salience selection during the  attention-demanding tasks  (e.g., attentional, expectational, perceptual and  working memory tasks). Dr.  Wang’s other focus is synaptic plasticity and its  regulation. He investigated  how the disease-linked mutant proteins may affect  AMPA and NMDA receptor-mediated  transmissions, and lead to defects in synaptic  plasticity, learning and  memory. He explored the manipulations that may reverse  the impaired  cognition. Hitherto, Dr. Wang has investigated CaV3.2 channel relating to childhood  absence epilepsy,  BRAG1 relating to X-linked mental disorder, DISC1 relating to  schizophrenia,  and FXR2P relating to fragile X syndrome. His work built the  foundation for  developing effective therapies for these cognitive  disorders. Dr.  Wang has joined the School of Life Sciences and Technology of  Harbin  Institute of Technology in December, 2017.

 

Research Interests

My  current research focuses on medial entorhinal cortex (MEC). MEC is the interface  between hippocampus and neocortex, and is believed to play an important role in  spatial navigation, learning, and memory. Actually MEC has been reported to  contain diverse spatial cell types, including grid cells, head direction cells,  border cells, and speed cells. Grid cells, which have multiple firing fields  forming a hexagonal grid, are the most important spatial cells. Since first  discovered in rats, grid cells have also been found in mice, bats, monkeys and  humans. Together with place cells in hippocampus, grid cells form a  comprehensive positioning system, an inner GPS, in the brain, while other  spatial cells, especially head direction and speed cells, are believed to offer  information to grid cells to perform path integration. In the past decade, in  vivo recordings of grid and other spatial cells deepened our understanding of  them, but how the grid firing fields form is still an enigma. Answering this  question requires not only more detailed behavior studies but also a  comprehensive knowledge of cellular properties and neuronal circuits in MEC.  Therefore, my interest is to reveal cellular and circuitry fundamentals of  spatial cells in MEC. Specifically, I will investigate the mechanism of grid and  other spatial cells using multiple independent approaches combining  electrophysiology, optogenetics, two-photon imaging, molecular biology, and  pharmacology, and focus on the following three aims:

Aim 1: To investigate cell types and local circuitry in  MEC.

Aim 2: To investigate the dorsal-ventral organization of cells and  circuits in MEC.

Aim 3: To investigate the afferent inputs from visual cortex to  MEC.

      Moreover, I will also develop in vivo recording system of  spatial cells, employing tetrode, juxtacellular, and/or fluorescence recordings.  After establishing the recording system, I will long to study the effect of  external sensory clues and environmental spatial frequency on spatial cells. In  addition, I am also interested in developing and applying new optical  technology, especially techniques combining nanoscience. By attaching gold  nanoparticles on the cellular surface, neurons can be activated optically in  subcellular resolution. Combined with intracellular nanoprobes, surface-enhanced  Raman spectroscopy has been used to distinguish different cell types of neurons.  I am eager to see whether these new approaches can assist my neuronal circuitry  study in finding neural connections and determining cell types of  interneurons.

 

Techniques and Tools in the Lab

Simultaneous multiple whole-cell recording

Morphological reconstruction of neurons

Two-photon  Ca2+/Na+ imaging

One-/two-photon optogenetic stimulation

Viral tools (Sindbis virus, lentivirus, AAV)

Fluorescence sensors (acetylcholine, serotonin, dopamine, glucose,  etc.)


Selected Publications(# Co-first  author; *Corresponding  author)

  1. Lim  CS, Wen C, Sheng Y,Wang  G,  Zhou Z, Wang S, Zhang H, Ye A, Zhu JJ (2017) Piconewton-Scale Analysis of  Ras-BRaf Signal Transduction with Single-Molecule Force  Spectroscopy.Small:DOI: 10.1002/smll.201701972. [IF: 8.643]
    Reported as news byPhys.org  (https://phys.org/news/2017-09-technique-doctors-disease-severity.html) andCBS  (http://www.newsplex.com/content/news/UVA-researchers-develop-new-molecule-measuring-technique-444053003.html).

  2. Wang G,  Bochorishvili G, Chen Y, Salvati KA, Zhang P, Dubel SJ, Perez-Reyes E, Snutch  TP, Stornetta RL, Deisseroth K, Erisir A, Todorovic SM, Luo JH, Kapur J,  Beenhakker MP, Zhu JJ (2015) CaV3.2  calcium channels control NMDA receptor-mediated transmission: a new mechanism  for absence-like epilepsy.Genes  Dev29:1535-1551.  [IF: 9.413]

    See commentary inEpilepsy  Currents16:36–38.

  3. Wang  G#,  Wyskiel DR#,  Yang W, Wang Y, Milbern LC, Lalanne T, Jiang X, Shen Y, Sun Q-Q and Zhu JJ  (2015) An optogenetics- and imaging-assisted simultaneous multiple patch-clamp  recordings system for decoding complex neural circuits.Nat  Protoc10:  397-412. [IF: 10.032]

    Highlighted as the cover article byNature Protocols.

  4. Lee  AJ#,Wang  G#,  Jiang X#,  Johnson SM, Hoang ET, Lanté F, Stornetta RL, Beenhakker MP, Shen Y, Zhu JJ  (2015) Canonical Organization of Layer 1 Neuron-Led Cortical Inhibitory and  Disinhibitory Interneuronal Circuits.Cereb  Cortex25:2114-2126.  [IF: 6.559]

  5. Wang G and  Zhu JJ (2014) DISC1 dynamically regulates synaptic N-methyl-D-aspartate  responses in excitatory neurons.Biol  Psychiatry75:  348-350. [IF: 11.412]

    Invited commentary.

  6. Jiang  X#,Wang  G#,  Lee AJ, Stornetta RL and Zhu JJ (2013) The Organization of Two New Cortical  Interneuronal Circuits.Nat  Neurosci16:  210-218. [IF: 17.839]

    See news and views inNature  Neurosci16:114-5;  highlights inCurr  Opin Neurobiol 26:7-14,Curr  Opin Neurobiol 26:15-21,Curr  Opin Neurobiol26:117-124,  andCurr  Opin Neurobiol32:107-14.

  7. Myers  KR#,Wang  G#,  Sheng Y, Conger KK, Casanova JE and Zhu JJ (2012) Arf6-GEF BRAG1 Regulates  JNK-Mediated Synaptic Removal of GluA1-Containing AMPA Receptors: A New  Mechanism for Nonsyndromic X-Linked Mental Disorder.J  Neurosci32:  11716-11726. [IF: 5.988]

  8. Ye  A andWang  G*  (2008) Dipole polarizabilities ofns21S0 andnsnp3P0 states  and relevant magic wavelengths of group-IIB atoms.Phys  Rev A78:  1-4 (Article #: 014502). [IF: 2.925]

  9. Wang Gand  Ye A (2007) Possibility of using Zn as the quantum absorber for a laser-cooled  neutral atomic optical frequency standard.Phys  Rev A76:  1-12 (Article #: 043409). [IF: 2.925]

  10. WangG,  Wen C and Ye A (2006) Dynamic holographic optical tweezers using a  twisted-nematic liquid crystal display.J  Opt A8:  703-708. [IF: 1.742]