Research


http://hcls.hit.edu.cn/_upload/article/images/96/38/7c408e6c4d769e4c112f7b41be59/c08a8405-53c5-4caf-a88a-de3419c2efda.jpgZhiwei  Huang: PhD, Professor

Phone:  86-0451-86403163

Email: huangzhiwei@hit.edu.cn;

huangzhiwei2009@gmail.com

Research  Area: Structural  Biology and

Signal Transduction


Lab Introduction: 

Dr. Zhiwei Huang is a professor of School of Life Science and Technology, Harbin Institute of Technology. After finishing his PhD thesis from Jijie Chai’s laboratory at National Institute of Biological Sciences in 2008, he went to Laurie H. Glimcher’s laboratory at Harvard University to receive his postdoctoral training. In March 2012 he joined the School and Life Science and Technology at Harbin Institute of Technology as professor.

Research Description

We use multi-disciplinary approaches including structural biology, biochemistry, virology, cell biology, and mouse genetics in our studies. Our research interests focus on:

1). Investigates the structure-function relationships of macromolecules (soluble & membrane proteins) of interest in the field of immunology and infectious diseases. Our research focus on understanding the molecular basis of important pathogen-host interactions. We are interested in studying structure-function relationship of soluble/membrane protein complexes involved in immunology and infectious diseases signaling pathways and using structural information to rational design therapeutic agents for the treatment of diseases.

2). Studies the molecular mechanisms of CRISPR-Cas systems. Most of archaea and many bacteria encode a diverse set of CRISPR-Cas systems as an adaptive immune system to defend themselves against phage infection. After integration of short segments of invader-derived DNAs (or RNAs in some cases) into a CRISPR array within the host genome, expression and processing of the precursor CRISPR RNAs (crRNAs) produces mature crRNAs. The mature crRNAs then guide an effector protein or a Cas protein complex, to target and cleave foreign DNAs (or RNAs in some cases) bearing complementary sequences. We are interested in figuring out how CRISPR-Cas systems work, and engineering CRISPR-Cas systems for precisely genome editing applications.

3). Identifies regulators and signal transduction events involved in cell death during HIV or bacterial infection. Pyroptosis is triggered by various pathological stimuli, such as pathogen infection, which is important for controlling microbial infection. But it is largely unknown for the mechanism on this pathogen-induced lytic form of cell death. Our efforts are focused on identifying novel signaling components and signaling pathways in pyroptosis.

Publications (* Co-first author, # Corresponding author

1.De Dong*, Kuan Ren*, Xiaolin Qiu*, Jianlin Zheng, Minghui Guo, Xiaoyu Guan, Hongnan Liu, Ningning Li, Bailing Zhang, Daijun Yang, Chuang Ma, Shuo Wang, Dan Wu, Yunfeng Ma, Shilong Fan, Jiawei Wang, Ning Gao and Zhiwei Huang#2016. Crystal structure of CRISPR-Cpf1 in complex with CRISPR RNA (crRNA). Nature. 532, 522-526.

  1. Yingying Guo*,      Liyong Dong*, Xiaolin Qiu*, Yishu Wang, Bailing Zhang, Hongnan Liu, You      Yu, Yi Zang, Maojun Yang and Zhiwei      Huang# (2014). Structural basis for hijacking      CBF-β and CUL5 E3 ligase complex by HIV-1 Vif. Nature. 505, 229-233.      (This paper is featured with News & Views, Nature. 505, 167-168,      highlighted in Nature      Structural & Molecular Biology, 21, 117 (2014))

  2. Jae-Hyuck Shim,      Matthew B. Greenblatt, Weiguo Zou, Zhiwei      Huang, Marc N. Wein, Nicholas Brady, Dorothy Hu, Jean      Charron, Heather R. Brodkin, Gregory A. Petsko, Dennis Zaller, Bo Zhai,      Steven Gygi, Laurie H. Glimcher and Dallas C. Jones (2013). Schnurri-3      regulates ERK downstream of WNT signaling in osteoblasts. J Clin Invest.      doi:10.1172/JCI69443.

  3. Zehan Hu, Chuangye Yan, Peiyuan Liu, Zhiwei Huang, Rui Ma, Chenlu Zhang, Ruiyong Wang, Yueteng Zhang, Fabio Martinon, Di Miao, Haiteng Deng, Jiawei Wang, Junbiao Chang, Jijie Chai (2013). Crystal structure of      NLRC4 reveals its autoinhibition mechanism. Science. 12, 172-175.

  4. Weiguo Zou, Xi      Chen, Jae Shim, Zhiwei      Huang, Nicholas Brady, Dorothy Hu, Rebecca Drapp, Kirsten      Sigrist, Laurie H. Glimcher, Dallas Jones (2011). The E3 ubiquitin ligase      Wwp2 regulates craniofacial development through monoubiquitination of      Goosecoid. Nature      Cell Biology. 13, 59-65.

  5. Chen D, Lei L,      Flores R, Zhiwei      Huang, Wu Z, Chai J, Zhong G. (2010). Autoprocessing and      self-activation of the secreted protease CPAF in Chlamydia-infected cells.      Microbial Pathogene­sis.      1(10), 1-10.

  6. Zhiwei Huang and Jijie Chai (2010). Mapping the selection mechanisms by      bacterial GEFs. Virulence.      1(2), 1-4.

  7. Zhiwei Huang*, Sarah E. Sutton*, Adam J. Wallenfang, Robert C. Orchard,      Xiaojing Wu, Yingcai Feng, Jijie Chai and Neal M. Alto (2009). Structural      insights into host GTPase isoform selection by a family of bacterial GEF      mimics. Nature Structural & Molecular Biology.      16(8), 853 – 860. (This publication was selected as cover story, and high­lighted      by Nature China

  8. Zhiwei Huang, Yingcai Feng, Ding Chen, Xiaojing Wu, Xiaojun Wang, Xingguo      Xiao, Wenhui Li, Niu Huang, Lichuan Gu, Guangming Zhong and Jijie Chai      (2008). Structural basis for activation and inhibition of the secreted      chlamydia protease CPAF. Cell      Host & Microbe. 4(6), 529-542. (This publication was      selected as a research “highlight” by Nature China

  9. Maikke B. Ohlson,      Zhiwei Huang, Neal M.      Alto, Jack E. Dixon, Jijie Chai and Samuel I. Miller (2008). Structure and      Function of Salmonella SifA Indicate that Its Interactions with SKIP,      SseJ, and RhoA Family GTPases Induce Endosomal Tubulation. Cell Host & Microbe.      4(5), 434-446